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题名:	Polycomb-Dependent Chromatin Looping Contributes to Gene Silencing during Drosophila Development
作者:	Ogiyama, Yuki Schuettengruber, Bernd Papadopoulos, Giorgio L. 張家銘 Chang, Jia-Ming Cavalli, Giacomo
贡献者:	資訊科學系
关键词:	3D genome;TADs;chromosome organization;chromatin;Hi-C;Drosophila embryogenesis;polycom;blooping interactions
日期:	2018-07
上传时间:	2018-07-24 17:29:42 (UTC+8)
摘要:	Interphase chromatin is organized into topologically associating domains (TADs). Within TADs, chromatin looping interactions are formed between DNA regulatory elements, but their functional importance for the establishment of the 3D genome organization and gene regulation during development is unclear. Using high-resolution Hi-C experiments, we analyze higher order 3D chromatin organization during Drosophila embryogenesis and identify active and repressive chromatin loops that are established with different kinetics and depend on distinct factors: Zelda-dependent active loops are formed before the midblastula transition between transcribed genes over long distances. Repressive loops within polycomb domains are formed after the midblastula transition between polycomb response elements by the action of GAGA factor and polycomb proteins. Perturbation of PRE function by CRISPR/Cas9 genome engineering affects polycomb domain formation and destabilizes polycomb-mediated silencing. Preventing loop formation without removal of polycomb components also decreases silencing efficiency, suggesting that chromatin architecture can play instructive roles in gene regulation during development.
關聯:	Molecular Cell, Volume 71, Issue 1, Pages 73-88.e5
数据类型:	article
DOI 連結:	https://doi.org/10.1016/j.molcel.2018.05.032
DOI:	10.1016/j.molcel.2018.05.032
显示于类别:	[資訊科學系] 期刊論文

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