政大機構典藏-National Chengchi University Institutional Repository(NCCUR):Item 140.119/70089
English  |  正體中文  |  简体中文  |  Post-Print筆數 : 27 |  全文笔数/总笔数 : 113303/144284 (79%)
造访人次 : 50796282      在线人数 : 641
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    请使用永久网址来引用或连结此文件: https://nccur.lib.nccu.edu.tw/handle/140.119/70089


    题名: Protein Kinase CK2 Impairs Spatial Memory Formation through Differential Cross Talk with PI-3 Kinase Signaling: Activation of Akt and Inactivation of SGK1
    作者: 趙知章
    Chao,Chih Chang;Ma,Yun L.;Lee,Eminy H. Y.
    贡献者: 神科所
    关键词: protein kinase CK2;serum- and glucocorticoid-inducible kinase 1;Akt;protein phosphatase 2A;spatial memory formation;hippocampus
    日期: 2007.06
    上传时间: 2014-09-23 12:25:30 (UTC+8)
    摘要: Casein kinase II (CK2) is a multifunctional serine/threonine protein kinase that is associated with the development of neuritogenesis and synaptic plasticity. The phosphoinositide 3-kinase (PI-3K)/Akt pathway is implicated in long-term memory formation. In addition, serum- and glucocorticoid-inducible kinase 1 (SGK1) is another downstream target of PI-3K signaling that was shown to play an important role in spatial memory formation. Whether CK2 may also affect memory formation and whether CK2 interacts with Akt and SGK1 during this process is unknown. In the present study, we found that water maze training significantly decreased CK2 activity in the rat hippocampal CA1 area but not inthe dentate gyrus (DG) area. Transfection ofthe dominant negative mutant of CK2, CK2 A156,tothe CA1 area, but not to the DG area, decreased CK2 activity but enhanced spatial memory formation. Meanwhile, it increased SGK1 phosphorylation at Ser422, decreased Akt phosphorylation at Ser473, and increased cAMP response element-binding protein phosphorylation at Ser133. Transfection ofthe constitutively active SGK1, SGKS422D, enhanced whereastransfection ofthe wild-type Aktimpaired spatial memory formation. Also, administration of the protein phosphatase 2A inhibitor, fostriecin, reversed the memory-impairing effect of CK2 WT. It also reversed the effect of CK2 WT in decreasing SGK1 phosphorylation. Akt Ser473 phosphorylation was moderately increased by CK2 WT and fostriecin treatment, but AktS473A mutant transfection reversed the memory-impairing effect of CK2 WT. These results together suggest that CK2 impairs spatial memory formation through differential cross talk with PI-3 kinase signaling by activation of Akt and inactivation of SGK1 through protein phosphatase 2A.
    關聯: The Journal of Neuroscience,27(23),6243-6248
    数据类型: article
    DOI 連結: http://dx.doi.org/10.1523/JNEUROSCI.1531-07.2007
    DOI: 10.1523/JNEUROSCI.1531-07.2007
    显示于类别:[神經科學研究所] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    6243-6248.pdf364KbAdobe PDF2973检视/开启


    在政大典藏中所有的数据项都受到原著作权保护.


    社群 sharing

    著作權政策宣告 Copyright Announcement
    1.本網站之數位內容為國立政治大學所收錄之機構典藏，無償提供學術研究與公眾教育等公益性使用，惟仍請適度，合理使用本網站之內容，以尊重著作權人之權益。商業上之利用，則請先取得著作權人之授權。
    The digital content of this website is part of National Chengchi University Institutional Repository. It provides free access to academic research and public education for non-commercial use. Please utilize it in a proper and reasonable manner and respect the rights of copyright owners. For commercial use, please obtain authorization from the copyright owner in advance.

    2.本網站之製作，已盡力防止侵害著作權人之權益，如仍發現本網站之數位內容有侵害著作權人權益情事者，請權利人通知本網站維護人員(nccur@nccu.edu.tw)，維護人員將立即採取移除該數位著作等補救措施。
    NCCU Institutional Repository is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff(nccur@nccu.edu.tw). We will remove the work from the repository and investigate your claim.
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈