 |
English
|
正體中文
|
简体中文
|
Post-Print筆數 : 27 |
Items with full text/Total items : 113648/144635 (79%)
Visitors : 51573887
Online Users : 939
|
|
|
Loading...
|
Please use this identifier to cite or link to this item:
https://nccur.lib.nccu.edu.tw/handle/140.119/65314
|
| Title: | Effects of surface charge and particle size of cell-penetrating peptide/nanoparticle complexes on cellular internalization. |
| Authors: | Liu, Betty Revon;Chan, Ming-Huan;Chen, Hwei-Hsien;Lo, Shih-Yen;Huang, Yue-Wern;Lee, Han-Jung
詹銘煥 |
| Contributors: | 神科所 |
| Date: | 2013.09 |
| Issue Date: | 2014-04-11 18:53:03 (UTC+8) |
| Abstract: | Cell membranes are natural barriers that prevent macromolecules from permeating cells. The efficiency of exogenous materials entering cells relies on various strategies and factors. Cell-penetrating peptides (CPPs) are distinctive molecules that can penetrate cells by themselves, as well as carry cargoes into cells in both covalent and noncovalent manners. In this chapter, we use CPP-mediated delivery of nanomaterials to illustrate the importance of surface charge and size of nanoparticles on cellular uptake. We found that three different arginine-rich CPPs (SR9, HR9, and PR9) are able to form stable complexes with nanomaterials, including quantum dots (QDs) and DNAs, and the complexes can effectively internalize into cells. Our study demonstrated that zetapotential of CPP/cargo nanoparticulate complexes is a key predictor of transduction efficiency. On a different note, a combination of CPPs with cargoes resulted in complexes with various sizes. The most positively charged HR9/cargo complexes displayed the highest protein transduction efficiency. The correlation coefficient analysis demonstrated a high correlation between zeta-potential and transduction efficiency of CPP/DNA complexes. A logarithmic curve was plotted with zeta value against transduction efficiency with an R-squared value of 0.9454. With similar surface charges, particle sizes could affect cellular uptake efficiency of CPP/QD complexes. Collectively, our findings elucidate that zeta-potential of CPP/cargo nanoparticulate complexes plays a major role in determining transduction efficiency, while particle sizes of CPP/cargo nanoparticulate complexes have a minor effect in cell permeability. |
| Relation: | Cell Membrane: Molecular Structure, Physiochemical Properties and Interactions with the Environment., pp.43-57
Nova Science Publishers , 2013
ISBN: 9781628084573 |
| Data Type: | book/chapter |
| Appears in Collections: | [神經科學研究所] 專書/專書篇章
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| 4357.pdf | | 1549Kb | Adobe PDF2 | 1315 | View/Open |
|
All items in 政大典藏 are protected by copyright, with all rights reserved.
|
著作權政策宣告 Copyright Announcement1.本網站之數位內容為國立政治大學所收錄之機構典藏，無償提供學術研究與公眾教育等公益性使用，惟仍請適度，合理使用本網站之內容，以尊重著作權人之權益。商業上之利用，則請先取得著作權人之授權。
The digital content of this website is part of National Chengchi University Institutional Repository. It provides free access to academic research and public education for non-commercial use. Please utilize it in a proper and reasonable manner and respect the rights of copyright owners. For commercial use, please obtain authorization from the copyright owner in advance.
2.本網站之製作，已盡力防止侵害著作權人之權益，如仍發現本網站之數位內容有侵害著作權人權益情事者，請權利人通知本網站維護人員(
nccur@nccu.edu.tw)，維護人員將立即採取移除該數位著作等補救措施。
NCCU Institutional Repository is made to protect the interests of copyright owners. If you believe that any material on the website infringes copyright, please contact our staff(
nccur@nccu.edu.tw). We will remove the work from the repository and investigate your claim.
