Loading...
|
Please use this identifier to cite or link to this item:
https://nccur.lib.nccu.edu.tw/handle/140.119/65235
|
| Title: | Astrgaloside IV increases MMP-2 mRNA and protein expression in human lung cancer A549 cells. |
| Authors: | Su, Chin-Cheng;Chiou, Tsrong-Laang;Chan, Ming-Huan;Lin, Jaung-Geng
詹銘煥 |
| Contributors: | 神科所 |
| Date: | 2009.01 |
| Issue Date: | 2014-04-09 17:43:57 (UTC+8) |
| Abstract: | Lung cancer is the leading cause of cancer-related death in Taiwan. In the clinical treatment of lung cancer patients in Asia, an Astragalus-based herbal mixture is commonly used in conjunction with chemotherapy. The principal component of Astragalus (also known as Huang-qi) is Astragaloside IV. The Astragaloside IV marker has been qualified for the phytochemicals of Astragalus. The recurrence and metastasis of lung cancer are positively correlated with matrix metalloproteinase-2 (MMP-2) mRNA and protein expression. This study examined the effects of Astragaloside IV on the mRNA and protein expression of MMP-2 in lung cancer A549 cells by RT-PCR and Western blotting. The cytotoxicity of Astragaloside IV in these cells was determined using CellTiter 96® AQueous One Solution Reagent and the MTT assay. A549 cells were treated for different durations with Astragaloside IV concentrations of 10, 20, 50 and 100 ng/ml. The respective proliferation rates per amount (relative to the control) were as follows: 24 h, 96.85±1.12, 95.63±0.83, 93.92±0.84, 95.27±0.57%; 48 h, 98.86±1.56, 95.71±0.59, 94.09±0.68, 93.44±0.5%; 72 h, 99.48±0.16, 95.60±0.48, 95.23±0.67, 94.72±1.12%. MMP-2 mRNA expression as well as vascular endothelial growth factor mRNA expression were upregulated at concentrations of 10, 20 and 50 ng/ml. Additionally, protein expression of MMP-2 was increased at concentrations of 10, 20 and 30 µg/ml after 24 h of treatment. These results indicate that Astragaloside IV upregulates MMP-2 mRNA and protein expression in A549 cells, and therefore that it may increase recurrence and metastatic rates in lung cancer. This issue should be further examined in the clinical setting. |
| Relation: | Molecular Medicine Report,2(1), 107-113 |
| Data Type: | article |
| DOI 連結: | http://dx.doi.org/10.3892/mmr_00000070 |
| DOI: | 10.3892/mmr_00000070 |
| Appears in Collections: | [神經科學研究所] 期刊論文
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| 107113.pdf | | 550Kb | Adobe PDF2 | 1573 | View/Open |
|
All items in 政大典藏 are protected by copyright, with all rights reserved.
|
