政大機構典藏-National Chengchi University Institutional Repository(NCCUR):Item 140.119/64582

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政大機構典藏 > 商學院 > 風險管理與保險學系 > 期刊論文 > Item 140.119/64582

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题名:	Unique marker finder algorithm generates molecular diagnostic markers
作者:	Chiu, Sung-Kay;Hsieh, Ming-Hua;Tzeng, Chi-Meng 謝明華;曾驥孟
贡献者:	風管系
关键词:	comparative genomics;subtraction;diagnostic markers;micro-array;real-time PCR.
日期:	2011-03
上传时间:	2014-03-11 17:38:43 (UTC+8)
摘要:	By taking advantage of the power of comparative genomics, we devised an algorithm, Unique Marker Finder (U-MarFin), to generate a collection of unique DNA sequences from a target organism. The whole target genome is partitioned into a scoring pool of less 4000 base-pair fragments, which are then subjected to elimination of homologous sequences in other bacterial genomes by BLAST alignment, and looked for all open reading frames as they may be applied as unique markers. Through regular, nested, multiplex and real time PCR and microarray technology, we empirically demonstrated that the sequences discovered were highly specific to the species that they are derived from, and they can serve as molecular biomarkers for diagnostic purpose.
關聯:	International Journal of Bioinformatics Research and Applications, 7(1), 24-42
数据类型:	article
DOI 連結:	http://dx.doi.org/10.1504/IJBRA.2011.039168
DOI:	10.1504/IJBRA.2011.039168
显示于类别:	[風險管理與保險學系] 期刊論文

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