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    题名: 總體基因Hi-C交互作用圖之網路分析與其組裝
    The network analysis of the metagenomic Hi-C contact map and its downstream metagenome assemble binning
    作者: 許育庭
    Hsu, Yu-Ting
    贡献者: 張家銘
    Chang, Jia-Ming
    許育庭
    Hsu, Yu-Ting
    关键词: Hi-C
    總體基因組裝基因組
    網路模型
    社群發現
    Hi-C
    Metagenome-assembled genomes
    Network models
    Community detection
    日期: 2022
    上传时间: 2022-10-05 09:14:07 (UTC+8)
    摘要: 背景：總體基因組學是對微生物群體進行基因分析研究，相對於傳統總體基因組分裝，近來利用染色體構象捕獲技術進行恢復個別物種的總體基因組，可以得到更好的分裝結果。在先前鄭惟文的碩士論文「HiCBin: 利用 Hi-C 交互網路對總體基因組裝進行反捲積」中，以bin3C的流程為主並提出了一個利用智慧局部移動法（SLM）分群的基因組分裝方法。
    結果：除了利用Hi-C資料進行基因組分裝，我們對總體基因組Hi-C連結網路進行分析，並發現在高品質的網路有較多的小世界網路特性，於是我們利用這些特性進行分裝網路品質的預測。此外我們也以bin3C的流程，並替換其他不同的社群發現演算法，去測試是否改善分群結果，而調整解析度後的SLM在兩個資料中表現較好。
    結論：我們的研究主要依據先前碩士論文但針對網路做更多分析，並多測試了三個資料集。雖較難得出以何種分群方法更好，但對於網路特性的發現可以為未來的研究提供一個新的觀點。實驗原始碼可以於以下連結中取得： https://github.com/changlabtw/Bin3C_SLM。
    Background: Metagenomics is the genomic analysis of microbial communities. Current approaches to metagenome-assembled-genomes (MAGs) recovery draw on chromosome conformation capture techniques and have been shown to outperform traditional genome binning methods. In the previous Cheng’s thesis, `HiCBin: Deconvoluting metagenomic assemblies via Hi-C connect networks`, she based on bin3C pipeline and described a Hi-C-based metagenomic deconvolution method using smart local movement algorithm (SLM) for genome binning.
    Results: In addition to using Hi-C data for genome binning, we further analyze the contact networks of metagenomic Hi-C and discover that the networks get higher quality to have more small-world characteristics. Therefore, we use the properties to predict the qualities of the clusters. We also follow the bin3C process and replace the clustering step with different community detection algorithms to check if it improves the outcome. SLM performs better in two datasets after adjusting the resolution parameter.
    Conclusion: In this work, we mainly followed Cheng’s thesis but did more analyses on the metagenomic Hi-C networks and tested three more datasets. Though it is hard to conclude a better cluster algorithm from this work, the discovery of the network properties might provide a new aspect for future works. The source code for the experiments is publicly available at https://github.com/changlabtw/Bin3C_SLM.
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    描述: 碩士
    國立政治大學
    資訊科學系
    108753127
    資料來源: http://thesis.lib.nccu.edu.tw/record/#G0108753127
    数据类型: thesis
    DOI: 10.6814/NCCU202201535
    显示于类别:[資訊科學系] 學位論文

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