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| Title: | 透過 Cp190和CTCF的缺失HiC實驗了解建築蛋白在果蠅染色體三結構角色
Loss of Cp190 and CTCF defines a genomic map of architectural elements in Drosophila Genomics of Drosophila architectural elements |
| Authors: | 鄺芷君
Kuong, Chi-Kuan |
| Contributors: | 張家銘
Chang, Jia-Ming
鄺芷君
Kuong, Chi-Kuan |
| Keywords: | 建築蛋白
絕緣子
architectural proteins
Hi-C
insulator |
| Date: | 2020 |
| Issue Date: | 2020-10-05 15:17:05 (UTC+8) |
| Abstract: | 在哺乳類動物中，TAD的邊界上富集了CTCF與cohesin的複合物。然而科學家發現在果蠅身上並未能找到CTCF/cohesin, 而富集在果蠅的TAD邊界上更多的是BEAF-32/CP190 或是 BEAF-32/Chromator。本文透過利用各種資料視覺化方法呈現建築蛋白以及與其結合位點間的相互作用與關係，從而推論昆蟲和哺乳動物是否通過不同的機制來構建TAD，結構蛋白對於昆蟲和哺乳動物基因組的折疊是否同樣重要。
在這次研究中使用了Chip-Seq，Hi-C和RNA-Seq數據，並且開發了相應的算法以證明在果蠅身上的絕緣子蛋白結合機制中，CP190在招募其他絕緣子並形成絕緣複合體中擔任了關鍵角色。
CTCF enriched around Mammals’ TAD boundaries, colocalized with cohesin complex, is one of Chromatin architectural proteins. However, instead of the absence of CTCF/cohesin, BEAF-32/CP190 and BEAF-32/Chromator were found at Drosophila’s TAD boundaries. The architectural proteins and their binding sites were used to probe into the relationships between architectural proteins via various visualization data approaches. And extending a provocative question of whether architectural proteins are equally important for proper folding of the insect and vertebrate genomes. Here, we analyzed Chip-Seq, Hi-C, and RNA-Seq data and developed a couple of analysis tools to identify the insulator protein binding mechanism in Drosophila. Cp190 is shown as a critical player in recruiting other insulators and forming an insulation complex. |
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| Description: | 碩士
國立政治大學
資訊科學系
107753045 |
| Source URI: | http://thesis.lib.nccu.edu.tw/record/#G0107753045 |
| Data Type: | thesis |
| DOI: | 10.6814/NCCU202001731 |
| Appears in Collections: | [資訊科學系] 學位論文
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